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![Western Blot: Parvalbumin Antibody [NB120-11427] - WB analysis of rat cerebellum extract.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Western-Blot-NB120-11427-img0002.jpg "Western Blot: Parvalbumin Antibody [NB120-11427] - WB analysis of rat cerebellum extract.")
![Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody [NB120-11427] - Analysis of Parvalbumin using anti-Parvalbumin polyclonal antibody shows staining in U251 Cells. Parvalbumin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Parvalbumin at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11427-img0008.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NB120-11427] - Stargazin expression is restricted to PV+ interneurons in WT mouse somatosensory cortex (upper row). Somatodendritic stargazin immunoreactivity is lost in PV+ interneurons in stargazer mouse (lower row). Co-labeling of stargazin and parvalbumin antibodies shown in layer 2/3 at 63x magnification (scale = 10 um). Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2013.00156/abstract), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NB120-11427-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody [NB120-11427] - Analysis of Parvalbumin using anti-Parvalbumin polyclonal antibody shows staining in C6 Cells. Parvalbumin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Parvalbumin at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11427-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody [NB120-11427] - Analysis of Parvalbumin using anti-Parvalbumin polyclonal antibody shows staining in Hela Cells. Parvalbumin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Parvalbumin at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11427-img0007.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NB120-11427] - Normal biopsies of deparaffinized human cerebellum tissue.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NB120-11427-img0003.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NB120-11427] - Normal biopsies of deparaffinized human skeletal muscle tissue.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NB120-11427-img0004.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NB120-11427] - Normal biopsies of deparaffinized human tonsil tissue.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NB120-11427-img0005.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NBP2-33529] - Analysis in human parathyroid gland and liver tissues. Corresponding Parvalbumin RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NBP2-33529-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Parvalbumin Antibody [NBP2-33529] - Analysis in human parathyroid gland and liver tissues. Corresponding Parvalbumin RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Parvalbumin Antibody [NBP2-33529] - Analysis in human cell line RPMI-8226.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Western-Blot-NBP2-33529-img0015.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NBP2-33529] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NBP2-33529-img0014.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NBP2-33529] - Staining of human parathyroid gland shows high expression.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NBP2-33529-img0008.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NBP2-33529] - Staining of human cerebellum shows strong positivity in Purkinje cells.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NBP2-33529-img0011.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NBP2-33529] - Staining of human cerebral cortex shows strong positivity in a small subset of neurons.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NBP2-33529-img0012.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NBP2-33529] - Staining of human kidney shows strong positivity in cells in distal tubules.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NBP2-33529-img0013.jpg)
![Western Blot: Parvalbumin Antibody (6C7K6) [NBP3-15664] - Analysis of extracts of various cell lines, using Parvalbumin (PVALB) antibody (NBP3-15664) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-6C7K6-Western-Blot-NBP3-15664-img0004.jpg "Western Blot: Parvalbumin Antibody (6C7K6) [NBP3-15664] - Analysis of extracts of various cell lines, using Parvalbumin (PVALB) antibody (NBP3-15664) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: Parvalbumin Antibody (6C7K6) [NBP3-15664] - Mouse brain using Parvalbumin (PVALB) Rabbit mAb (NBP3-15664) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-6C7K6-Immunohistochemistry-Paraffin-NBP3-15664-img0003.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody (6C7K6) [NBP3-15664] - Rat brain using Parvalbumin (PVALB) Rabbit mAb (NBP3-15664) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-6C7K6-Immunohistochemistry-Paraffin-NBP3-15664-img0001.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody (6C7K6) [NBP3-15664] - Human appendix using Parvalbumin (PVALB) Rabbit mAb (NBP3-15664) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-6C7K6-Immunohistochemistry-Paraffin-NBP3-15664-img0002.jpg)
![Western Blot: Parvalbumin Antibody [NB100-1541] - (0.01ug/ml) staining of Human Cerebellum (A) lysate + peptide (B) and (0.3ug/ml) Rat Brain (C) lysate + peptide (D) (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Western-Blot-NB100-1541-img0002.jpg "Western Blot: Parvalbumin Antibody [NB100-1541] - (0.01ug/ml) staining of Human Cerebellum (A) lysate + peptide (B) and (0.3ug/ml) Rat Brain (C) lysate + peptide (D) (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Flow Cytometry: Parvalbumin Antibody [NB100-1541] - Analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Flow-Cytometry-NB100-1541-img0001.jpg)